PCR Supplies
Epoch Life Science Inc GenCatch™ Advanced PCR Cleanup Kit (250 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
PCR purification kit. Silica membrane spin column based. Sample volume: 850 ul. Column binding capacity: 40 ug DNA. Minimum elution volume: 20ul. 18 month shelf life.
Takara Bio LENTI-X QRT-PCR TITRATION
Retroviral and lentiviral titration kits that measure virus titers using SYBR Green-based, real-time PCR. Viral copy numbers are determined by comparing the sampleAEs Ct to a standard curve derived from a calibrated control template.
New England Biolabs, Inc. WarmStart RTx Reverse Transcriptase – 250 reactions
WarmStart RTx Reverse Transcriptase is a unique in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40C. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in LAMP (loop-mediated isothermal amplification). The WarmStart property enables high throughput applications, room temperature setup, and increases the consistency and specificity of amplification reactions. RTx contains intact RNase H activity.
New England Biolabs, Inc. Bst 2.0 DNA Polymerase – 1600 units
Bst 2.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment). Bst 2.0 DNA Polymerase contains 5' to 3' DNA polymerase activity and strong strand displacement activity but lacks 5' to 3' exonuclease activity. Bst 2.0 DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
Takara Bio PrimeSTAR® HS DNA Polymerase (premix), 100 Rxns
A 2X hot-start PCR master mix containing Takara's high-fidelity PrimeSTAR HS DNA polymerase, optimized reaction buffer, and dNTPs. PrimeSTAR HS DNA polymerase has superior proofreading ability due to robust 3' to 5' exonuclease activity. It can efficiently amplify up to 8.5 kb for human genomic DNA targets or up to 22 kb for lambda DNA. Please refer to the User Manual for premix composition. 100 Rxns
Lonza Walkersville MgCl2 10 mM Solution
MgCl2 can be used as the sample diluent when attempting to overcome inhibitory chelation effect. Examples of samples which chelate divalent cations include heparin and EDTA. MgCl2 may be used to prepare samples for any LAL assay. Contains <0.005 EU/ml endotoxin.Content:30 mL/vial
101 BIO 2x Es Taq Master Mix (with dye) (1 mL, 100 rxn, 20 ul/rxn)
This Master Mix contains Es Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer. The concentration is 2x.Es Taq DNA Polymerase possesses 5´→3´ DNA polymerase and 5´→3´ exonuclease activity. The polymerase has the high amplification efficiency and low mismatching as Taq DNA polymerase and high fidelity of Pfu DNA polymerase. Es Taq Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for T/A cloning. The amplification range of Es Taq is ~ 6 kb.This unique Master Mix recipe makes the system very reliable. More than 98% of PCR reaction can get successful amplification during the first try. It also works well on complicate templates.The Master Mix contains dye, and can directly run electrophoresis after PCR reaction.
Invivogen TLRL-PICW, Short Synthetic Analog of dsRNA, 25 mg
Short synthetic analog of dsRNA, size 25 mg. Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection. Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production. Poly(I:C) is recognized by Toll-like receptor 3 (TLR3) [1, 2]. Upon poly(I:C) recognition, TLR3 activates the transcription factor interferon regulatory factor 3 (IRF3), through the adapter protein Toll-IL-1 receptor (TIR) domain-containing adapter inducing IFN-b (TRIF, also known as TICAM-1) [3]. Activation of IRF3 leads to the production of type I IFNs, especially IFN-b. A second pathway involves the recruitment of TNF receptor-associated factor 6 (TRAF6) or receptor interacting protein 1 (RIP1), with the subsequent activation of the transcription factors NF-B and AP-1 [4].
Leishmania tarentolae Parrot (strain Tar II), frozen axenic culture for research (ATCC 30267)
Leishmania tarentolae Parrot (strain Tar II) is a saurian-associated parasitic protozoan provided as a frozen, cryopreserved culture for laboratory research. The strain is maintained axenically and is suited to molecular biology, parasitology, and recombinant protein expression workflows.
- Axenic parasitic protozoan strain isolated from Tarentola mauritanica (lizard).
- Supplied cryopreserved for long-term storage and recovery to culture.
- Cultured in diphasic blood agar medium; incubate at 25°C with the cap secured.
- Biosafety level 1 handling requirements.
- Suitable as a nonpathogenic model for Leishmania biology and protein expression studies.
Agilent Technologies PFUTURBO CX DNA POLY 100 UNITS
PfuTurbo Cx Hotstart DNA Polymerase, 100U, Specially formulated with a mutant of Pfu DNA polymerase designed to overcome uracil sensitivity for more robust performance. Stored at -20degreeC